Comparative Evaluation of Marking Effects of Two Fluorescent Chemicals, Alizarin Red S and Calcein, on Black Sea Bream (Acanthopagrus schlegelii)

Two fluorescent dyes, alizarin red S (ARS) and calcein (CAL), were applied to evaluate the marking effects on juveniles of Acanthopagrus schlegelii. The total mortality rates experimental groups significantly lower (p < 0.05) than those control groups, but no significant difference was detected between two staining methods. fluorescence microscopy observation results showed that quality ARS better CAL, with fin spines rays being best tissues. optimal concentration for CAL 200 mg/L 350 mg/L, respectively. To ensure mark quality, recommended dye grade above 3, most suitable conditions suggested be labeling at a immersion 24 h. will provide useful data information future research stock enhancement using chemical method.